兰花种类不同，适于种子发芽的培养基也有所区别。一种培养基对某些种类发芽是有效的，但对于别的种类则效果不佳或无效。对培养基的配制，几十年来，国外有大量报道。但对于我国兰花种类，特别是地生兰的种子发芽的培养基，则还有待于摸索和研究。

    兰属植物种子发芽的培养基有20多种，兹介绍几种比较常用的如下。在组织培养中的 MS培养基也可用于种子播种。

    l、Knudson Sol.N培养基              （g）

    Ca（NO3）2·4H2O硝酸钙                 1

    （NH4）2SO4硫酸铵                      0.5
 
    MgSO4·7H2O硫酸镁                      0.25

    KH2PO4磷酸二氢钾                       0.025

    FeSO4·7H2O硫酸亚铁                    0.05

    琼脂                                    15

    蔗糖                                    20

    蒸馏水                                 1000ml

    pH5.l

    2、Mariat F．1952培养基              （g）

    MgSO4·7H2O酸酸镁                       0.25

    Ca（NO3）2·4H2O硝酸钙                    1

    KH2PO4磷酸二氢钾                         0.25

    CaCl2氯化钙                              0.12

    FeC13氯化铁                              0.001

    蔗糖                                      20

    琼脂                                      16

    蒸馏水                                   1000ml

    pH4.8—5.2

    3、Mariat F培养基                    （g）

    MgSO4·7H2O硫酸镁                         0.25

    KNO3硝酸钾                                0.25

    Ca（NO3）2·4H2O硝酸钙                      1

    KH2PO4磷酸二氢钾                           0.11

    K2HPO4磷酸氢钾                             0.14

    FeC13氯化铁                                微量

    K2SiO2硅酸钾                               微量

    蔗糖                                        20

    琼脂                                        16

    重蒸水                                     1000ml

    pH4.8－5.2

    4、Knudson C培养基                    （g）

    Ca（NO3）2·4H2O硝酸钙                        1

    KH2PO4磷酸二氢钾                             0.25

    FeSO4·7H2O硫酸亚铁                          0.025

    （NH4）2SO4硫酸铵                             0.5

    MgSO4·7H2O硫酸镁                             0.2

    MnSO4·4H2O硫酸锰                            0.0075

    蔗糖                                          2％

    琼脂                                         1.5％

    pH5.1l

     5、Vacin et Went（vw）培养基              （g）

    Ca3（PO4）2磷酸钙                             0.200

    KNO3硝酸钾                                    0.525 

    KH2PO4磷酸二氢钾                              0.25

    MgSO4·7H2O硫酸镁                             0.25

    （NH4）2SO4硫酸铵                              0.5

    Fe（C4H4O6）3酒石酸铁                          0.028

    MnSO4·4H2O硫酸锰                              0.0075

    糖                                              2％

    pH5—5.2

    6、Thomale 1954培养基                    （g）

    （NH4）2SO4硫酸铵                               0.06

    （NH4）NO3硝酸铵                                0.37

    KNO3硝酸钾                                      0.4

    KH2PO4磷酸二氢钾                                 0.3

    Mg（NO3）2硝酸镁                                 0.11

    FeSO4· 7H2O硫酸亚铁                             0.02

    琼脂                                              16

    果糖                                              10

    葡萄糖                                             10

    蒸馏水                                           1000ml

    pH4.8-5.1

                                                       吴应祥

（记者 佚名）